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Cryopreservation of Spermatozoa from Closed Colonies, and Inbred, Spontaneous Mutant, and Transgenic Strains of Rats

Authors: Nakatsukasa, Ena1; Kashiwazaki, Naomi2; Takizawa, Akiko2; Shino, Masao2; Kitada, Kazuhiro3; Serikawa, Tadao4; Hakamata, Yoji5; Kobayashi, Eiji5; Takahashi, Riichi6; Ueda, Masatsugu6; Nakashima, Tatsuyuki7; Nakagata, Naomi7

Source: Comparative Medicine, Volume 53, Number 6, December 2003 , pp. 639-641(3)

Publisher: American Association for Laboratory Animal Science

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Abstract:

We attempted to cryopreserve spermatozoa from closed colonies (Jcl:SD and Jcl:Wistar), and inbred (BN/Crj, F344/DuCrj, LEW/Crj, Long-Evans and WKY/NCrj), mutant (Zitter [WTC.ZI-zi] and Tremor [TRM]), transgenic (human A-transferase [A], and green fluorescent protein [GFP]) strains of rats. Rat epididymal spermatozoa suspended in cryopreservation solution (23% egg yolk, 8% lactose monohydrate, and 0.7% Equex Stm, pH 7.4, adjusted with 10% Tris [hydroxymethy] aminomethane) were frozen and stored at –196°C. After thawing at 37°C, the spermatozoa were instilled into the tip of each uterine horn of the recipients. A total of five recipient females for each strain were inseminated with cryopreserved spermatozoa, and normal live offspring of all strains (Jcl:SD: 11, Jcl:Wistar: 13, BN/Crj: 9, F344/DuCrj: 28, LEW/Crj: 4, Long-Evans: 6, WKY/NCrj: 8, TRM: 24, WTC.ZI-zi: 27, A: 30 and GFP: 20) were obtained.

Document Type: Research Article

Affiliations: 1: Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan, Trans Genic Inc., Kumamoto, Japan 2: Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan 3: Laboratory of Mammalian Genetics, Center for Advanced Science and Technology, Hokkaido University, Hokkaido, Japan 4: Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan 5: Division of Transgenic Animal Research, Center for Molecular Medicine, Jichi Medical School, Tochigi, Japan 6: YS New Technology Institute Inc., Shimotsuga, Tochigi, Japan 7: Center for Animal Resources and Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan

Publication date: December 1, 2003

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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