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Open Access Effect of Animal Bedding on Rat Liver Endosome Acidification

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Animal beddings, such as pine products, and environmental factors are known to induce liver drug-metabolizing cytochrome P450 enzymes. We observed that a change to pine-based rat bedding altered baseline and cAMP-stimulated rates of acidification in rat liver endosomes, apparently by decreasing ATP-dependent proton transport in the presence and absence of chloride. Although cAMP altered phosphorylation of protein kinase B and extracellular signal-regulated kinases 1 and 2 (ERK 1,2) and p38 mitogen-activated protein kinases, changes in housing conditions did not affect baseline or cAMP-stimulated values of these or other selected signaling molecules. We conclude that compounds in rat bedding may alter not only drug metabolism, but also aspects of endocytosis.

Document Type: Research Article

Affiliations: 1: Medical Service, 11R Research Service, Ann Arbor VA Hospital, 2215 Fuller Road, Ann Arbor, Michigan 48105 2: Medical Service, 11R Research Service, Ann Arbor VA Hospital, 2215 Fuller Road, Ann Arbor, Michigan 48105, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109

Publication date: December 1, 2003

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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