Study of Low-Temperature (4°C) Transport of Mouse Two-Cell Embryos Enclosed in Oviducts
Abstract:Purpose: We examined usefulness of a mouse embryo transportation system for low-temperature transport of oviducts containing mouse two-cell embryos.
Methods: Oviducts containing two-cell mouse embryos were stored at 4°C for 36 h. After that, embryos were collected and cultured for 96 h in Potassium Simplex Optimized Medium (KSOM) medium and evaluated for their rate of development to hatched blastocysts. Embryos were transferred to recipients, and the rate of survival to live young was investigated. The oviducts were then transported from Yamagata to Kumamoto (distance of approx. 1,000 km). At the destination, embryos were implanted in recipient dams and were studied to evaluate their survival to live young.
Results: After preservation for 36 h at 4°C, 68.3% of two-cell embryos developed to hatched blastocysts. As a result of transplanting 546 embryos into 25 recipients, 109 normal live young mice were obtained; the rate of development was 20.0%. Results of oviduct transport from Yamagata to Kumamoto indicated that 30.2% of transplanted embryos developed to live young.
Conclusion: Low-temperature transport of two-cell embryos in oviducts is useful as a method of shipping mouse embryos between institutes.
Document Type: Research Article
Affiliations: 1: Laboratory Animal Center, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata ,990-9585, Japan 2: Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, 2-2-1 Honjo, Kumamoto, 860-0811, Japan
Publication date: August 1, 2003
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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