Development and Use of a Simple Polymerase Chain Reaction Assay to Screen for Helicobacter spp. and H. hepaticus in Intestinal and Fecal Samples from Laboratory Mice
Abstract:A simple and sensitive duplex polymerase chain reaction (PCR) assay was developed for use in detection of Helicobacter species and H. hepaticus in laboratory mice. Bacteria were extracted and concentrated from fecal pellets and intestinal segments by use of buoyant density centrifugation. To improve quality assurance, an internal control (mimic) for detection of false-negative reactions was included. In addition, cartridges (Capillette) pre-filled with PCR reagents, were used to minimize the hands-on time required, thus reducing the risk of contamination with previously amplified material. Laboratory mice from Swedish animal houses sent to the National Veterinary Institute for health monitoring were found to have high prevalence of H. hepaticus.
Document Type: Research Article
Affiliations: 1: National Veterinary Institute, SE-751 89 Uppsala, Sweden; CanAg Diagnostics AB, Majnabbe Terminal, SE-414 55 Gothenburg, Sweden 2: National Veterinary Institute, SE-751 89 Uppsala, Sweden
Publication date: 2002-12-01
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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