Delivery of Deoxyribonucleic Acid (DNA) to Somatic Cells: An Overview of Species and Strain-related Responses
Abstract:There is great potential in successful delivery of genetic material to somatic cells of animals and humans, not only to treat genetic deficiencies, but also to provide more effective therapies and vaccines. Concerted effort has been mounted over the past decade to validate the concept of gene-based therapy. Delivery of genes in vitro via multiple methods has demonstrated the biologic usefulness of the basic concept. However, application of similar gene delivery strategies to whole animal systems has been more difficult. Much of the complexity associated with gene delivery involves encounters with host biological barriers, including innate and acquired host responses to exogenous DNA, as well as specifically encoded proteins. Delivery of genetic material to somatic cells in vivo is a multi-factorial event involving variables in formulation, route, and dose, and target species or strain. In vivo expression of these variables can result in conflicting findings with regard to gene expression and toxicity. These findings may compromise the predictive reliability of interstrain and, particularly, interspecies studies. This review will address representative in vivo somatic cell transfection modalities, variability in species and strain responses following delivery of nucleic acids, and some of the potential mechanisms involved.
Document Type: Research Article
Affiliations: Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, Washington, 98195
Publication date: December 1, 2002
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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