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Open Access Comparative Study of Histopathologic Characterization of Azoxymethane-induced Colon Tumors in Three Inbred Rat Strains

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To obtain controlled genetic variation, colon cancer was chemically induced by use of four subcutaneous injections of azoxymethane (15 mg/kg of body weight/wk) to rats of 3 inbred strains (BDIX/OrlIco, F344/NHsd, WAG/Rij). The selection was based on the availability of established colon cancer cell lines arising from these particular strains. In the first experiment, only female rats were used; in the second experiment, both sexes were studied. The goal was to select a rat strain giving the highest tumor frequency with the shortest latency period in reproducible manner. The histologic characteristics should resemble the corresponding human tumors. The size of the tumors should be at about 1 cm in diameter, as these tumor cells were intended to be used in future transplantation studies. The two experiments yielded highly reproducible results: histologic evaluation of all colon tumors in all three rat strains revealed adenomas and adenocarcinomas closely resembling their human counterpart. The BDIX strain had the highest tumor frequency (75%) in both sexes and the shortest minimal latency period (28 weeks in experiment 1; 23 weeks in experiment 2). Tumor size of about 1 cm in diameter was found most often in the BDIX strain. On the basis of results of these two experiments, the BDIX strain has been selected for future study.

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Document Type: Research Article

Affiliations: 1: Biomedical Laboratory, Faculty of Health Sciences, University of Southern Denmark - Odense, Winsløwparken 23, DK-5000 Odense C, Denmark 2: Department of Pathology, Faculty of Health Sciences, University of Southern Denmark - Odense, Winsløwparken 15, DK-5000 Odense C, Denmark 3: Novo Nordisk, Novo Allé, DK-2880 Bagsværd, Denmark 4: Department of Laboratory Animal Science, Utrecht University, Yalelaan 2, 3584CM Utrecht, The Netherlands 5: Department of Statistics and Demography, Faculty of Health Sciences, University of Southern Denmark - Odense, Sdr. Boulevard 23A, DK-5000 Odense C, Denmark

Publication date: 01 February 2002

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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