Intestinal Cannulation: Model for Study of the Midgut of the Pig
Abstract:Purpose: To develop a pig model that would enable repeated biopsy specimen collection and endoscopic monitoring of the gut. This would increase precision of the experiment and reduce the number of experimental animals required.
Methods: Six 10-week-old Yorkshire pigs underwent surgery, and a cannula was inserted in the cecum. Two pigs served as non-operated controls. The health status of the animals was monitored by clinical, hematologic, and biochemical examinations and by studies of gut motility and microbial flora. The experimental period lasted for eight weeks and approximately 45 biopsy specimens were obtained from each animal.
Results: Repeated endoscopy was performed and biopsy specimens were taken. Adverse effects on the animal's health were not apparent, and differences were not evident in transit time of digesta or in diversity of the gut microbial flora. After surgery there was a transient increase in the concentrations of haptoglobin, serum amyloid A, and plasma cortisol, and in body temperature and white blood cell count.
Conclusions: It is possible to use an intestinal cannula in the cecum both for endoscopy and biopsy specimen collection. The procedures did not influence health status of the pigs, nor alter gut function. The method will be useful in experimental infection studies as well as in other physiologic investigations.
Document Type: Research Article
Affiliations: 1: Department of Large Animal Clinical Sciences, National Veterinary Institute, Uppsala, Sweden 2: Department of Animal Nutrition and Management, National Veterinary Institute, Uppsala, Sweden 3: Department of Pathology, National Veterinary Institute, Uppsala, Sweden 4: Department of Clinical Chemistry, Swedish University of Agricultural Sciences, National Veterinary Institute, Uppsala, Sweden
Publication date: April 1, 2001
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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