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Open Access Mamu -DQA1 Allele and Genotype Frequencies in a Randomly Sampled Breeding Colony of Rhesus Macaques (Macaca mulatta)

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Abstract:

We studied the allelic and genotypic distribution of the major histocompatibility class-II locus DQA1 observed in a random sample of Indian rhesus macaques (Macaca mulatta) from a major breeding facility in the United States. The DNA was isolated from whole blood samples collected between 1991 and 1994 from 65 Indian rhesus monkeys. Polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP), which involves use of specific amplification of DQA1 exon 2 and subsequent restriction digestion of the 242-base pair fragment, was used to genotype the animals for the 20 known macaque (Mamu)-DQA1 alleles. Frequencies for four alleles (DQA1*240x, *2502, *2503 and *0102) differed significantly from those reported in a smaller sample of rhesus macaques from the German Primate Center. The modest genetic survey of Mamu-DQA1 genotypes presented here will be particularly useful in designing epidemiologic studies that investigate associations between immunogenetic background and disease susceptibility in macaque models of human disease.

Document Type: Research Article

Affiliations: 1: Departments of Medicine and Epidemiology, School of Veterinary Medicine, University of California at Davis, California 2: Coriell Institute for Medical Research, Camden, New Jersey 3: Department of Veterinary Pathology, Purdue University, West Lafayette, Indiana 4: Departments of Medicine and Epidemiology, School of Veterinary Medicine, University of California at Davis, California, California Regional Primate Research Center, Davis, California 5: California Regional Primate Research Center, Davis, California, Department of Anthropology, University of California, Davis, California.

Publication date: April 1, 2001

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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