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Open Access Behavioral, Clinical, and Physiologic Analysis of Mice Used for Ascites Monoclonal Antibody Production

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Background and Purpose: The effects of pristane inoculation, ascites accumulation, peritoneocentesis, and analgesics on the well-being of mice used in monoclonal antibody (MAb) production protocols were investigated.

Methods: Four experiments, each containing 17 to 21, 6- to 8-week-old male Balb/c mice, were conducted. Each experiment involved a period in which baseline data were collected, followed by intraperitoneal injections of pristane or phosphate-buffered saline (PBS) inoculations into each mouse. One week later mice received intraperitoneal inoculations of either hybridoma cells or PBS. Parameters used to assess well-being throughout each of these periods included: wheel-running activity, food and water consumption, open-field box activity, clinical observation, and plasma corticosterone concentration.

Results: Compared to controls, pristane inoculation had slight to no affect on mice. There was no evidence of distress in cell-inoculated mice prior to their gaining 25% of their baseline body weight. The number of times (up to three) that peritoneocentesis was performed did not have a significant impact on mice's well-being, but ascites yields were greater when multiple harvests were performed. Cell-inoculated mice that gained weight slowly or developed high-particulate ascites were at higher risk of being distressed.

Conclusion: Ascites yields can be maximized by performing multiple harvests; however, the well-being of mice used in such protocols should be closely monitored, as suggested here.

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Document Type: Research Article

Publication date: 2000-10-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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