Behavioral, Clinical, and Physiologic Analysis of Mice Used for Ascites Monoclonal Antibody Production
Methods: Four experiments, each containing 17 to 21, 6- to 8-week-old male Balb/c mice, were conducted. Each experiment involved a period in which baseline data were collected, followed by intraperitoneal injections of pristane or phosphate-buffered saline (PBS) inoculations into each mouse. One week later mice received intraperitoneal inoculations of either hybridoma cells or PBS. Parameters used to assess well-being throughout each of these periods included: wheel-running activity, food and water consumption, open-field box activity, clinical observation, and plasma corticosterone concentration.
Results: Compared to controls, pristane inoculation had slight to no affect on mice. There was no evidence of distress in cell-inoculated mice prior to their gaining 25% of their baseline body weight. The number of times (up to three) that peritoneocentesis was performed did not have a significant impact on mice's well-being, but ascites yields were greater when multiple harvests were performed. Cell-inoculated mice that gained weight slowly or developed high-particulate ascites were at higher risk of being distressed.
Conclusion: Ascites yields can be maximized by performing multiple harvests; however, the well-being of mice used in such protocols should be closely monitored, as suggested here.
Document Type: Research Article
Publication date: 2000-10-01
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
Attention Members: To access the full text of the articles, be sure you are logged in to the AALAS website.
Attention: please note, due to a temporary technical problem, reference linking within the content is not available at this time
- Editorial Board
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- For issues prior to 1998
- Institutional Subscription Activation
- Ingenta Connect is not responsible for the content or availability of external websites