Poor Quality of Oocytes from Xenopus laevis Used in Laboratory Experiments: Prevention by Use of Antiseptic Surgical Technique and Antibiotic Supplementation
Methods: After a long phase of poor oocyte quality at our facility, involving oocytes that had been obtained by use of a “clean” surgical procedure, we subsequently cultured oocytes in a buffer medium containing the three antibiotics: penicillin G, gentamicin, and streptomycin.
Results: During DNA injection experiments, the oocytes developed black spots on their surface by postoperative day two. Pure culture of the gram-negative non-fermentative rod Pseudomonas fluorescens was obtained from the impaired oocytes; the isolate was resistant to the three antibiotics. By contrast, after aseptic surgical removal and culture of oocytes in buffer medium containing the antibiotics tetracycline and gentamicin, perfect oocytes without bacterial contamination were obtained.
Conclusion: Whenever impaired oocyte quality is observed, microbial contamination should be considered as a possible cause.
Document Type: Research Article
Publication date: 2000-04-01
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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