@article {Thorn:2000:1532-0820:153,
	author = "Thorn, Catherine E. and Papp, John R. and Shewen, Patricia E. and Stirtzinger, Tatiana",
	title = "Experimentally Induced Pneumonia in scid/beige Mice, Using a Bovine Isolate of Pasteurella haemolytica",
	journal = "Comparative Medicine",
	volume = "50",
	number = "2",
	year = "2000",
	abstract = "<I>Background and Purpose:</I> Intranasal challenge of immunocompetent mice with <I>Pasteurella haemolytica</I> results in little or no pulmonary inflammation. The study reported here was designed to investigate the inflammatory response in the lungs of immunodeficient scid/beige mice after similar challenge.<P></P> <I>Methods:</I> Fifty-five <I>scid/beige</I> mice were challenged intranasally with saline or one of three doses (2.8 x 10<SUP>6</SUP>, 3.4 x 10<SUP>9</SUP>, or 3.3 x 10<SUP>11</SUP> colony-forming units [CFU]/ml) of <I>P. haemolytica</I>. The lungs were examined for changes in weight, bacterial count, and presence of gross and microscopic lesions at 24, 48, or 96 hours after challenge.<P></P> <I>Results:</I> Intranasal challenge with concentrations &#8805;3.4 x 10<SUP>9</SUP> CFU/ml of P. haemolytica induced significantly heavier lung weight, with severe pulmonary lesions, and development of suppurative and fibrinous bronchopneumonia in dose- and time-dependent manner 48 hours after challenge. <I>Pasteurella haemolytica</I> was consistently isolated from the lungs at 24 hours after challenge.<P></P> <I>Conclusions:</I> Bronchopneumonia was induced by <I>P. haemolytica</I> in mice without manipulation of the mouse or the bacteria. The lesions were similar to those that develop in the lungs of cattle infected with <I>P. haemolytica</I> and indicate potential use of the model for the study of this host/bacterial interaction.",
	pages = "153-159",
	url = "http://www.ingentaconnect.com/content/aalas/cm/2000/00000050/00000002/art00009"
}