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Open Access Regional Variations in the Number and Subsets of Intraepithelial Lymphocytes in the Mouse Small Intestine

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Background and Purpose: Regional variations in the intraepithelial lymphocytes (IELs) in the mouse small intestine were examined.

Methods: The small intestine was taken from six 12-week-old BALB/c mice and divided equally into three parts (proximal, middle, and distal). The IELs were isolated from each part of the intestine and analyzed by use of flow cytometry.

Results: The number of IELs in the distal part of the small intestine was significantly lower than that in the proximal and middle parts (P < 0.01). The distribution of IEL subsets was markedly different between the proximal and the distal parts, and that in the middle part had an intermediate pattern. The percentage of T cells and expression of Thy-1.2 on the T and T cells were higher in the distal part (P < 0.01). The percentage of CD8 and of double-negative T cells were higher in the proximal part, whereas that of CD4CD8 double-positive T cells was higher in the distal part (P < 0.01). Regional variations were found mainly in the extrathymically developed T cell subsets.

Conclusion: Local development of IELs may differ between regions of the small intestine.

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Document Type: Research Article

Affiliations: 1: Department of Veterinary Public Health, The University of Tokyo, Tokyo, Japan 2: Faculty of Agriculture, The University of Tokyo, Tokyo, Japan

Publication date: 2000-02-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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