Embryonic Hematopoiesis Defect in the Tattered-Hokkaido (Tdho) Mouse
Methods: Suppression subtractive hybridization and Northern blot analysis were performed on E12.5 male Tdho and normal mouse embryos. In addition, histochemical examinations and blood cell counts of normal and mutant E12.5 embryos were carried out.
Results: Diminished expression of embryonic globin genes (and globins) in the blood of E12.5 Tdho male embryos were documented by use of Northern blot analysis and subtractive hybridization. Increased apoptosis of yolk sac-derived erythrocytes was found in E12.5 Tdho male embryos. Furthermore, diminished numbers of anucleated erythrocytes were observed in E12.5 mutant embryos.
Conclusions: A defect of embryonic hematopoiesis in Tdho mice was suggested as one of the possible causes of embryo lethality.
Document Type: Research Article
Affiliations: Laboratory of Experimental Animal Science, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
Publication date: 2000-02-01
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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