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Open Access Antibody Responses After Sendai Virus Infection and Their Role in Upper and Lower Respiratory Tract Disease in Rats

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Abstract:

Background and Purpose: Sendai virus infection in rats is an excellent model for studying development and role of host defenses throughout the respiratory tract after this infection. Therefore, development of serum antibody responses and disease were studied.

Methods: Forty-two anesthetized pathogen-free 3- to 4- week-old LEW/NCr rats were inoculated intranasally with Sendai virus. At postinoculation days 0, 2, 3, 5, 8, 10, and 14, rats were euthanized by administration of a pentobarbital sodium overdose followed by exsanguination. Serum was obtained from all animals, and nasal wash and bronchoalveolar lavage specimens were collected during selected experiments. An ELISPOT assay was used to measure numbers of Sendai virus-specific antibody-forming cells in respiratory tract lymphoid tissue.

Results: Recovery from disease and clearance of virus from respiratory tract tissues coincided with development of serum antibody responses. Upper respiratory tract lymph nodes were the initial and major sites of appearance of antibody-forming cells. Immunoglobulin G was the predominant subtype of these cells during recovery from the infection and in rats resistant to infection. Passive transfer of antisera or specific IgG protected the lower but not the upper respiratory tract.

Conclusions: Circulating components of immunity have a major role in resistance and recovery from disease in the lower respiratory tract, whereas local responses are likely involved in protection of the upper respiratory tract. Local lymphoid tissues are the major production sites of IgG, which contributes to resistance to and recovery from respiratory tract diseases.

Document Type: Research Article

Affiliations: 1: National Animal Center, National Defense Medical Center, P. O. Box 90048, Taipei, Taiwan, R.O.C 2: Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas 3: Department of Comparative Medicine, The University of Alabama at Birmingham, Medical Center, Birmingham, Alabama; and Division of Comparative Medicine, Department of Pathobiology, University of Florida, Gainesville, Florida 4: Department of Microbiology, The University of Alabama at Birmingham, Medical Center, Birmingham, Alabama; and Division of Comparative Medicine, Department of Pathobiology, University of Florida, Gainesville, Florida 5: The University of Alabama at Birmingham, Medical Center, Birmingham, Alabama; and Division of Comparative Medicine, Department of Pathobiology, University of Florida, Gainesville, Florida

Publication date: 1999-08-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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