Monoclonal Antibody Production in Murine Ascites II. Production Characteristics
Methods: Five hybridoma cell lines were grown in groups of 20 mice. Fourteen days prior to inoculation with 10 6 hybridoma cells, mice were primed with 0.5 ml of pristane given intraperitoneally. Ascites fluid was collected a maximum of three times by abdominal paracentesis; volume was measured and antibody concentration was determined by ELISA for each sample.
Results: Trends differed among cell lines when comparing ascites volumes and antibody concentrations over time from the first to the third tap. Antibody production was greatest at tap 1 for Groups 2B11 and 2C6D9; tap 2 for Group 3C9; and tap 3 for Groups RMK and 3D6. Total antibody production ranged from 422.90 to 996.64 mg; total ascites fluid volume ranged from 74.2 to 115.7 ml; and mean antibody concentration for taps 1, 2, and 3 ranged from 2.50 to 15.03 mg/ml among cell lines.
Conclusion: Production characteristics were significantly different among hybridoma cell lines. Determination of production characteristics of hybridomas and correlation with clinicopathologic changes in mice may be valuable in making recommendations for managing mice with ascites.
Document Type: Research Article
Affiliations: 1: Division of Comparative Medicine, Division of Toxicology, Massachusetts Institute of Technology, Cambridge, Massachusetts 2: Biogen, Inc., 14 Cambridge Center, Cambridge, MA 02142 3: Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, Memorial Sloan-Kettering Cancer Center and the Cornell University Medical College, 1275 York Avenue, Box 270, New York, NY 10021
Publication date: 01 February 1999
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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