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Open Access Monoclonal Antibody Production in Murine Ascites

I. Clinical and Pathologic Features

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Background and Purpose: Murine ascites production has been associated with appreciable morbidity and mortality, thus raising animal-welfare concerns. To address these concerns, the clinicopathologic changes associated with in vivo production of monoclonal antibodies in mice were characterized, and results were compared among cell lines.

Methods: Five hybridoma cell lines were grown in groups of 20 mice. Fourteen days prior to inoculation with 10 6 hybridoma cells, mice were primed with 0.5 ml of pristane given intraperitoneally; 12 mice were sham treated (controls). Ascites fluid was collected a maximum of three times by abdominal paracentesis. Clinical observations and pre- and postabdominal tap body weights were recorded. Necropsies were performed on all mice.

Results: For all groups combined, overall survival to tap 1 was 98%, to tap 2 was 96%, and to tap 3 was 79%; survival among groups ranged from 90 to 100% for tap 1, 85 to 100% for tap 2, and 35 to 100% for tap 3. Disseminated intra-abdominal seeding with irregular soft tissue and/or solid tumor masses was observed at necropsy.

Conclusions: Significant clinicopathologic changes were associated with monoclonal antibody production in mice, and differences between various hybridoma cell lines were apparent.
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Document Type: Research Article

Affiliations: 1: Division of Comparative Medicine, Division of Toxicology, Massachusetts Institute of Technology, Cambridge, Massachusetts 2: Biogen, Inc., 14 Cambridge Center, Cambridge, MA 02142 3: Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, Memorial Sloan-Kettering Cancer Center and the Cornell University Medical College, 1275 York Avenue, Box 270, New York, NY 10021

Publication date: 1999-02-01

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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