Latissimus Dorsi Cardiomyoplasty: A Chronic Experimental Porcine Model. Feasibility Study of Cardiomyoplasty in Danish Landrace Pigs and Göttingen Minipigs
Abstract:Cardiomyoplasty is an experimental treatment for end-stage heart failure. We hypothesized that the porcine latissimus dorsi muscle (LDM) in an experimental porcine model is a suitable surrogate for a clinically relevant evaluation of this concept. Fourteen Danish Landrace (DL) pigs and six Göttingen minipigs (GM) were studied. The LDM was evaluated immediately after surgical dissection and in various phases: phase 1 (n = 4)—outcome of a partial vascular isolation (vascular delay), 2 to 3 weeks prior to heart wrapping in DL pigs; phase 2 (n = 6)—long-term flap survival of nonstimulated LDM cardiomyoplasty in DL pigs; phase 3 (n = 6)—outcome of nonstimulated cardiomyoplasty in GM; phase 4—one DL pig had dynamic cardiomyoplasty performed and was subjected to low-intensity LDM stimulation for 8 months.
Isolation of the LDM of DL pigs and GM as a pedicled graft had no acute deleterious impact on the global blood supply. In phase 1a, partial vascular isolation and in situ recovery of the LDM resulted in a muscle encapsulated in fibrotic tissue, which hampered a later heart wrap. In phase 1b, a less extensive dissection diminished fibrosis and allowed subsequent wrapping. In phase 2, after 6 weeks of nonstimulated LDM cardiomyoplasty, the LDM of DL pigs was viable, with excellent heart-muscle integration. In phase 3, the same procedure applied in GM yielded the same result as that in DL pigs, but with a higher success rate owing to the learning phase.
Document Type: Research Article
Affiliations: 1: Department of Cardiothoracic & Vascular Surgery, Skejby Sygehus, Aarhus University Hospital, DK-8200 Aarhus N, Denmark, Institute of Experimental Clinical Research, Skejby Sygehus, Denmark 2: Department of Cardiothoracic and Vascular Surgery, Institute of Experimental Clinical Research, Skejby Sygehus, Denmark 3: Institute of Pathology, Aarhus Kommunehospital, Aarhus University Hospital, Denmark 4: Department of Cardiothoracic and Vascular Surgery, Denmark
Publication date: October 1, 1998
Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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