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Open Access Rapid Diagnosis of Simian Varicella Using the Polymerase Chain Reaction

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Abstract:

Simian varicella virus (SVV) causes sporadic epizootics of a varicella-like disease in nonhuman primates. Rapid diagnosis of simian varicella is critical in controlling epizootics. A polymerase chain reaction (PCR)-based diagnostic assay for detection of SVV DNA in cell culture and clinical samples from SVV-infected monkeys was developed. The assay is rapid, specific, and highly sensitive. The SVV DNA is readily detected in skin rash specimens and in peripheral blood lymphocytes of infected monkeys during the early stages of clinical varicella. In addition to providing an important diagnostic tool, the SVV PCR assay is also useful for investigating the epidemiology and pathogenesis of simian varicella.

Document Type: Research Article

Affiliations: 1: Department of Microbiology and Immunology, Slot 511, University of Arkansas for Medical Sciences, 4301 W. Markham Street, Little Rock, AR 72205 2: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 3: The Tulane Regional Primate Center, Tulane University, Covington, Louisiana

Publication date: February 1, 1998

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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